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ABSTRACT: Blood samples were obtained from 38 wild red deer (Cervus elaphus) at two sites in Ireland and subjected to PCR analysis of the 18S rRNA gene followed by sequencing. Two fragments of the 18S rRNA gene were generated by two different PCR protocols and subsequent sequencing suggested that at least six of the deer were infected by a babesia that, in those loci, is indistinguishable from Babesia divergens, an important tick-borne pathogen of cattle and of zoonotic significance. Additionally, a B. odocoilei-like parasite was detected in three samples and a babesia that did not match any sequences in the GenBank database was found in five samples. Neither B. capreoli nor B. venatorum (EU1) were found. There have been several reports of B. divergens occurring in deer species, including red deer, roe deer (Capreolus capreolus) and reindeer (Rangifer tarandus). However, in view of recent re-sequencing of bovine-origin samples deposited previously in GenBank, it is unlikely that any of these sequences from deer are B. divergens. The present study describes the only deer piroplasm detected so far that shows complete identity with B. divergens, in just over half of the 18S rRNA gene. The entire gene of this deer parasite should be analysed and transmission experiments undertaken before the infectivity of B. divergens for red deer can be confirmed.  相似文献   
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土壤重金属基线值的确定对于评价重金属积累现状具有重要意义。在存在人为污染的情况下,土壤重金属数据经常强烈地偏离正态分布和对数正态分布,影响统计分析结果的可信度,而稳健统计方法可以降低离群值对统计分析结果的影响。本研究以张家港为研究区域,采用稳健回归方法,建立表层和底层土壤Cu、Pb的回归关系,通过底层土壤Cu、Pb含量计算表层土壤Cu、Pb基线值,并对土壤Cu、Pb积累状况进行评价。结果表明,稳健回归方法确定的表层土壤Cu、Pb基线值可以反映出不同样点间,土壤Cu、Pb背景含量的自然变异。与稳健统计学方法相比,使用国家土壤环境质量标准值,或大区域(江苏省)的土壤背景值,评价自然背景本身存在变异区域的土壤重金属积累状况时,可能会产生较大的误差。  相似文献   
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Accurate, fine spatial resolution predictions of surface air temperatures are critical for understanding many hydrologic and ecological processes. This study examines the spatial and temporal variability in nocturnal air temperatures across a mountainous region of Northern Idaho. Principal components analysis (PCA) was applied to a network of 70 Hobo temperature loggers systematically distributed across 2 mountain ranges. Four interpretable modes of variability were observed in average nighttime temperatures among Hobo sites: (1) regional/synoptic; (2) topoclimatic; (3) land surface feedback; (4) canopy cover and vegetation. PC time series captured temporal variability in nighttime temperatures and showed strong relationships with regional air temperatures, sky conditions and atmospheric pressure. PC2 captured the topographic variation among temperatures. A cold air drainage index was created by predicting PC2 loadings to elevation, slope position and dissection indices. Nightly temperature maps were produced by applying PC time series back to the PC2 loading surface, revealing complex temporal and spatial variation in nighttime temperatures. Further development of both physically and empirically based daily temperature models that account for synoptic atmospheric controls on fine-scale temperature variability in mountain ecosystems are needed to guide future monitoring efforts aimed at assessing the impact of climate change.  相似文献   
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ABSTRACT

This paper reviews current developments that facilitate increased access to food science information, with examples from the Web, from search engine producers, and from disseminators and producers of specialised abstracting and indexing (A&I) databases. The diversity of developments is reviewed in the context of blogs, wikis, Facebook, YouTube, Connotea, and examples of increasing Web access with Google Scholar and Windows Live Search Academic are provided. Examples of A&I search platform developments which are discussed include CSA Illustrata, EBSCOhost visual search, and ISI Web of Knowledge. A simple case study is included which indicates user preferences for search engines, databases, and search interface features for accessing food science information. It is concluded that information providers are successfully using a number of development strategies designed to help the food science information community with its needs.  相似文献   
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As a consequence of the recent introduction of the pine wood nematode Bursaphelenchus xylophilus in Portugal, nematodes of the genus Bursaphelenchus were looked for in various French pine forests, in trees attacked by Monochamus galloprovincialis, the vector insect of B. xylophilus, and in the insects themselves. Trap trees were felled in 12 localities distributed all over the country. Nematodes were extracted from transversal stem discs; insects emerging from the trap trees were studied. B. hellenicus, B. leoni, B. mucronatus and B. sexdentati were isolated, but not B. xylophilus. The presence of B. mucronatus and the absence of B. xylophilus were confirmed by molecular markers. B. mucronatus was isolated from several regions with an average prevalence of 19%. The infestation of M. galloprovincialis by B. mucronatus reached 26.7%. The wide distribution of B. mucronatus in France could have an effect on the extension of B. xylophilus in a case of an introduction.  相似文献   
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